Nanomedicines Characterization Core Facility is part of the UNC Center for Nanotechnology in Drug Delivery (CNDD).

Nanoparticles fabrication:

• Nova™IJM: Advanced Lipid Nanoparticle (LNP) Manufacturing
• UnChained Labs Sunshine (providing capacities from in vitro to in vivo)

Exosomes isolation and characterization:

• Exosomes isolation from cells media/tissues (ExoQuick® ULTRA EV Isolation System, ultracentrifugation, size exclusion).
• Exosomal markers analysis (western blot, Exo-Check™ Exosome Antibody Array), Proteomics.
• Size, size distribution, concentration, surface charge, purity assessment: Zetaview (ParticleMetrix), Dynamic Light Scattering (DLS), Multisizer 4E Coulter Counter.

Analytical analysis:

• Size, size distribution, concentration, surface charge: Dynamic Light Scattering (DLS), Zetaview (ParticleMetrix), Multisizer 4E Coulter Counter.
• Materials analysis (GPC): weight average molar mass (Mw), number average molar mass (Mn), polydispersity, structure: Infra-red (IR), Thermogravimetric analysis (TGA).
• Analytical capabilities: High Performance Liquid Chromatography, UV-Vis spectroscopy, UV-temperature correlation
• Protein, peptides, RNA analysis: Capillary electrophoresis (protein purity, isoelectric focusing, Glycan analysis), Automated Western Blot Analysis (Protein Simple), Gel Imager.
• Elemental analysis: metals analysis in soils, water, food, plants, cells, tissues by ICP-MS.

In Vitro Assays:

• Protein concentration (BCA assay).
• Endotoxin contamination (LAL assay).
• In vitro stability analysis.
• In vitro drug release analysis.
• In vitro cellular uptake, transfection and toxicity analysis.

Data analysis and presentation:

• We offer expert opinion related to study design, sample selection/collection and storage, proposal review and development, manuscript development and review.

NCore collaborates with other Cores on UNC’s campus (TEM, Proteomics, Flow Cytometry, NMR, etc.) to facilitate comprehensive analysis of the samples.