FPLC (Fast Protein Liquid Chromatography) is a widely used analytical technique employed to separate polymers, proteins and other biomolecules such as nucleic acids. It features high loading capacity, biocompatible aqueous buffer systems, fast flow rates, and availability of stationary phases in most common chromatography modes (e.g., ion exchange, gel filtration, reversed phase, and affinity). It can be used to study composition of complex mixtures, for example: nanoparticles, products of bioconjugation reactions such as modified proteins, polypeptides and the like. Our technical capabilities with the AKTA FPLC system connected to the Knauer Model 2300 RI detector allow monitoring protein/peptide and polymer components in complex mixtures. Further, the FPLC is in-line with Zetasizer Nano ZS that can allow particle-sizing option of the different fractions present in the sample.
GPC (Gel Permeation Chromatography) is an analytical technique in which particles/polymers are separated based on their hydrodynamic volume. GPC/SEC is used to characterize natural and synthetic polymers, biopolymers, proteins and nanoparticles. GPC/SEC features both organic and aqueous mobile phase. Our technical capabilities include Malvern Viscotek TDAmax system connected to Tetra Detector Array (TDA), (RI, Light Scattering, Viscosity and UV detectors) and provide a distribution of absolute molecular weight, molecular size, and intrinsic viscosity as well as information on macromolecular structure, conformation aggregation and branching. Our system allows detection of nanoparticles as well as protein/peptide and/or polymer components in complex mixtures. Furthermore, the fractions separated by GPC can be collected and their chemical structure determined by IR/NMR.