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Capillary Electrophoresis

PA 800 Plus Pharmaceutical Analysis System (SCIEX, Framingham, MA)

  • Automated and quantitative analysis of biotherapeutics
  • Highly reproducible data

Key applications:

  • Quantitative protein purity analysis with SDS-Gel Capillary Electrophoresis
  • Advanced capillary isoelectric focusing and hi-speed Capillary Zone Electrophoresis for charge heterogeneity analysis
  • Glycan analysis
  • Peptide and protein biotherapeutics beyond mAbs (monoclonal antibodies)
  • Plasmid/Nucleic Acid purity, heterogeneity and sizing analysis

Western Blotting System

Wes (Protein Simple, San Jose, CA)

  • Highly sensitive automated Western blotting system
  • Can run 25 samples simultaneously
  • Can detect protein at the nanogram level in nanoliter-volume samples
  • Three-hour run time

Sample Required: 0.3-1.2 µg

Volume Required: 3 µL/well     

Size Range:  2-440 kDa   

Resolution:  ± 15-20% for MW <20 kDA, ± 10% for MW >20 kDa

Dynamic Range:    2-3 logs        

Sensitivity: ng       

Key applications:

  • Qualitative detection of single proteins and protein-modifications
  • Same-day verification of protein expression
  • Quantitative protein expression data