Electrophoresis
Capillary Electrophoresis
PA 800 Plus Pharmaceutical Analysis System (SCIEX, Framingham, MA)
- Automated and quantitative analysis of biotherapeutics
- Highly reproducible data
Key applications:
- Quantitative protein purity analysis with SDS-Gel Capillary Electrophoresis
- Advanced capillary isoelectric focusing and hi-speed Capillary Zone Electrophoresis for charge heterogeneity analysis
- Glycan analysis
- Peptide and protein biotherapeutics beyond mAbs (monoclonal antibodies)
- Plasmid/Nucleic Acid purity, heterogeneity and sizing analysis
Resources
https://www.youtube.com/watch?v=RYnGzlBc1rY
https://www.youtube.com/watch?v=xKTDKO2mM0Q
Western Blotting System
Wes (Protein Simple, San Jose, CA)
- Highly sensitive automated Western blotting system
- Can run 25 samples simultaneously
- Can detect protein at the nanogram level in nanoliter-volume samples
- Three-hour run time
Sample Required: 0.3-1.2 µg
Volume Required: 3 µL/well
Size Range: 2-440 kDa
Resolution: ± 15-20% for MW <20 kDA, ± 10% for MW >20 kDa
Dynamic Range: 2-3 logs
Sensitivity: ng
Key applications:
- Qualitative detection of single proteins and protein-modifications
- Same-day verification of protein expression
- Quantitative protein expression data