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Mass-spectroscopy

Inductively Coupled Plasma Mass-Spectrometer (ICP-MS)

NexION 300D (PerkinElmer, Inc., Waltham, MA)

  • Ability to analyze most of the frequently encountered elements in the periodic table (Mg, Al, P, Sc, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Ge, As, Zr, Mo, Ag, Cd, In, An, Ce, Gd, Tb, Ir, Pt, Au, Hg, Pb, Bi, and others)
  • Wide dynamic range: Part-per-trillion to high part-per-million
  • Only technique which can detect toxic heavy metals (As, Cd, Hg and Pb) as low as 10 pg/ml
  • Data system that allows corrections for isobaric interferences and the application of the internal standard technique

Resolution: less than or equal to 0.9 amu at 10% peak height from 6-253 amu and 1.0 amu at 5% peak height from 6-253 amu

Short-term precision: < 3% Relative Standard Deviation (RSD)

Long-term stability: < 4% RSD over 4 hours

Isotope-ratio precision: < 0.08*% RSD (*or within a factor of two of the counting statistics limit)

Mass calibration stability: < 0.05 amu over 8 hours of continuous operation

Key applications:

  • Concentration measurement of metal-containing proteins
  • Cell uptake studies
  • Drug loading/drug release
  • Biodistribution studies
  • Heavy metals analysis
  • Soil and water quality analysis
  • Food studies
  • Plant studies