Size, concentration and surface charge
Nanoparticle Tracking Analysis
ZetaView® QUATT (Particle Metrix Inc.)
- Instrument for measuring hydrodynamic particle size, zeta potential and concentration
- Enhanced fluorescence measurement capability
- Multi-wavelength F-NTA reduces total measurement time, minimizes the volume of sample required and improves experimental reproducibility
Laser wavelength: 405 nm, 488 nm, 520 nm, 640 nm
Key Applications
- Extracellular Vesicles
- Nanoparticles
- SPIONs
- Virus & Virus-Like-Particles (VLPs)
- Quantum-dots
NanoSight NS500 (Malvern Panalytical, Ltd, UK)
- Detailed and intuitive analysis of the size distribution and concentration
- Visual validation of results gives extra confidence
- Minimal sample preparation
Laser wavelength: 532 nm
Long pass fluorescent filter: 565 nm
Size range (diameter): 30 nm to 1000 nm
Concentration: 107 to 109 particles per ml
Sample volume: 20 – 100 ul
Key applications:
- Size distribution and polydispersity
- Sample concentration
- Protein aggregation studies
- Exosomes and microvesicles (extracellular vesicles) characterization
- Fluorescently labelled exosome and nanoparticles analysis
Dynamic Light Scattering/Zeta Potential meter
Zetasizer Nano ZS (Malvern Panalytical, Ltd, UK)
- Simple, rapid, repeatable and non-destructive technique
- Low volume sample measurement (for valuable proteins and biopolymer samples)
- Size measurement at low concentrations
Light Source: 633 nm
Accuracy: Better than +/-2% on NIST traceable latex standards 0.12µm·cm/V·s for aqueous systems using NIST, SRM1980 standard reference material
Size range (diameter): 0.3 nm to 10 μm
Size range for Zeta potential: 3.8 nm to 100 μm
Key applications:
- Proteins and polymer analysis
- Size determination
- Polydispersity index determination
- Zeta potential measurements